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Phospho-PKC Delta (Ser359) Polyclonal antibody

Phospho-PKC Delta (Ser359) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29562-1-AP

Print datasheet

Synonyms

MAY1, Phospho-PKC Delta (Ser359), PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

验证数据展示

  • WB analysis using 29562-1-AP

    WB analysis using 29562-1-AP

    Non-treated Jurkat, phosphatase inhibitor treated and λ phosphatase treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29562-1-AP (Phospho-PKC Delta (Ser359) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of A431 using 29562-1-AP

    IF Staining of A431 using 29562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated A431 cells using Phospho-PKC Delta (Ser359) antibody (29562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inλ phosphatase treated Jurkat cells
Positive IF/ICC detected inλ phosphatase treated A431 cells
Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29562-1-AP targets Phospho-PKC Delta (Ser359) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivitymouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, delta
Synonyms MAY1, Phospho-PKC Delta (Ser359), PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta
Calculated Molecular Weight 78 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. PKCδ, unlike other members of the PKC family, is unique in its regulation by tyrosine phosphorylation on multiple sites that determine activation, localization, and substrate specificity. PKCδ is activated by inflammatory mediators involved in the inflammatory response including lipopolysaccharide (LPS), tumor necrosis factor (TNF) and interleukin-1 (IL-1). PKCδ activation requires multi-phosphorylation steps which triggers translocation from the cell cytosol to different subcellular compartments. (PMID: 30095599, PMID: 31323909, PMID: 30917487)

实验方案

Product Specific Protocols
WB protocol for Phospho-PKC Delta (Ser359) antibody 29562-1-APDownload protocol
IF protocol for Phospho-PKC Delta (Ser359) antibody 29562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB

Cancers (Basel)

Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway

Authors - Qian Jiang
View Article