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验证数据展示

  • WB analysis using 29564-1-AP

    WB analysis using 29564-1-AP

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC Delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

Phospho-PKC Delta (Thr507) Polyclonal antibody

Phospho-PKC Delta (Thr507) Polyclonal Antibody for WB, ELISA
Cat No. 29564-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Phospho-PKC Delta (Thr505), Phospho-PKC Delta, Phospho-PKCδ, Phospho-PKCδ (Thr505), Phospho-PKCδ (Thr507)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPMA treated THP-1 cells, THP-1 cells

This antibody is equivalent to Thr505 in mice.

Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29564-1-AP targets Phospho-PKC Delta (Thr507) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, delta
别名 Phospho-PKC Delta (Thr505), Phospho-PKC Delta, Phospho-PKCδ, Phospho-PKCδ (Thr505), Phospho-PKCδ (Thr507)
计算分子量 78 kDa
观测分子量70 kDa
GenBank蛋白编号BC043350
基因名称 PKC Delta
Gene ID (NCBI) 5580
RRIDAB_3085346
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05655
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKC Delta(PRKCD) is a member of the PKC family, a phospholipid-dependent family of serine/threonine kinases, and is present in human neutrophils. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation. This antibody is equivalent to Thr505 in mice.

实验方案

Product Specific Protocols
WB protocol for Phospho-PKC Delta (Thr507) antibody 29564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols