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Phospho-PKC Delta (Thr507) Polyclonal antibody

Phospho-PKC Delta (Thr507) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29564-1-AP

Print datasheet

Synonyms

MAY1, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

验证数据展示

  • WB analysis using 29564-1-AP

    WB analysis using 29564-1-AP

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC Delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

经过测试的应用

Positive WB detected inPMA treated THP-1 cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29564-1-AP targets Phospho-PKC Delta (Thr507) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, delta
Synonyms MAY1, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta
Calculated Molecular Weight 78 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKC Delta(PRKCD) is a member of the PKC family, a phospholipid-dependent family of serine/threonine kinases, and is present in human neutrophils. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation.

实验方案

Product Specific Protocols
WB protocol for Phospho-PKC Delta (Thr507) antibody 29564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols