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Species Reactivity
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验证数据展示

  • WB analysis using 29564-1-AP

    WB analysis using 29564-1-AP

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC Delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

Phospho-PKC Delta (Thr507) Polyclonal antibody

Phospho-PKC Delta (Thr507) Polyclonal Antibody for WB, ELISA
Cat No. 29564-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-PKC Delta (Thr505), Phospho-PKC Delta, Phospho-PKCδ, Phospho-PKCδ (Thr505), Phospho-PKCδ (Thr507)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPMA treated THP-1 cells, THP-1 cells

This antibody is equivalent to Thr505 in mice.

Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29564-1-AP targets Phospho-PKC Delta (Thr507) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, delta
Synonyms Phospho-PKC Delta (Thr505), Phospho-PKC Delta, Phospho-PKCδ, Phospho-PKCδ (Thr505), Phospho-PKCδ (Thr507)
Calculated Molecular Weight 78 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
RRIDAB_3085346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKC Delta(PRKCD) is a member of the PKC family, a phospholipid-dependent family of serine/threonine kinases, and is present in human neutrophils. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation. This antibody is equivalent to Thr505 in mice.

实验方案

Product Specific Protocols
WB protocol for Phospho-PKC Delta (Thr507) antibody 29564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols