Phospho-PLA2G4A (Ser505) Polyclonal antibody
Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | λ phosphatase treated HeLa cells |
Positive IF/ICC detected in | λ phosphatase treated HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
Synonyms | cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A |
Calculated Molecular Weight | 749 aa, 85 kDa |
Observed Molecular Weight | 100-110 kDa |
GenBank Accession Number | BC114340 |
Gene Symbol | PLA2G4A |
Gene ID (NCBI) | 5321 |
RRID | AB_2918213 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P47712 |
Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and and 0.05%BSA. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-AP | Download protocol |
IF protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |