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Phospho-PLA2G4A (Ser505) Polyclonal antibody

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28925-1-AP

Print datasheet

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A

验证数据展示

  • WB analysis using 28925-1-AP

    WB analysis using 28925-1-AP

    Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

  • IF Staining of HeLa using 28925-1-AP

    IF Staining of HeLa using 28925-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inλ phosphatase treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
RRIDAB_2918213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47712
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and and 0.05%BSA.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)

实验方案

Product Specific Protocols
WB protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-APDownload protocol
IF protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols