Phospho-PLCG1 (Tyr783) Polyclonal antibody

Phospho-PLCG1 (Tyr783) Polyclonal Antibody for WB, ELISA
Cat No. 29566-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, ELISA

Phospholipase C gamma 1, Phospholipase C II, phospholipase C, gamma 1, PLC 148, PLC gamma 1, PLC II, PLC1, PLC148, PLCG1, PLCgamma1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inλ phosphatase treated Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29566-1-AP targets Phospho-PLCG1 (Tyr783) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, gamma 1
别名 Phospholipase C gamma 1, Phospholipase C II, phospholipase C, gamma 1, PLC 148, PLC gamma 1, PLC II, PLC1, PLC148, PLCG1, PLCgamma1
观测分子量150 kDa
GenBank蛋白编号BC144136
基因名称 PLCG1
Gene ID (NCBI) 5335
RRIDAB_2935418
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19174
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.

实验方案

Product Specific Protocols
WB protocol for Phospho-PLCG1 (Tyr783) antibody 29566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Commun Biol

FPR1 signaling aberrantly regulates S100A8/A9 production by CD14+FCN1hi macrophages and aggravates pulmonary pathology in severe COVID-19

Authors - Zhongyi Wang
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