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Phospho-PLCG2 (Tyr1217) Polyclonal antibody

Phospho-PLCG2 (Tyr1217) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29568-1-AP

Print datasheet

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, Phospho-PLCG2 (Tyr1217), PLC gamma 2, PLC IV, PLCG2

验证数据展示

  • WB analysis of Raji using 29568-1-AP

    WB analysis of Raji using 29568-1-AP

    Non-treated Raji cells and pervanadate treated Raji cells were subjected to SDS PAGE followed by western blot with 29568-1-AP (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

经过测试的应用

Positive WB detected inpervanadate treated Raji cells
Planning an IHC experiment? We recommend our IHCeasy PLCG2 Ready-To-Use IHC Kit. PLCG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29568-1-AP targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Synonyms Phospholipase C gamma 2, Phospholipase C IV, Phospho-PLCG2 (Tyr1217), PLC gamma 2, PLC IV, PLCG2
Calculated Molecular Weight 148 kDa
Observed Molecular Weight148-150 kDa
GenBank Accession NumberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)

实验方案

Product Specific Protocols
WB protocol for Phospho-PLCG2 (Tyr1217) antibody 29568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols