验证数据展示
经过测试的应用
| Positive WB detected in | pervanadate treated Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84456-1-RR targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phospholipase C, gamma 2 (phosphatidylinositol-specific) |
| 别名 | p- PLCγ2, p PLCγ2, p PLCG2 (Tyr1217), p PLCγ2 (Tyr1217), Phospho PLCG2 (Tyr1217) |
| 计算分子量 | 148 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC007565 |
| 基因名称 | PLCG2 |
| Gene ID (NCBI) | 5336 |
| RRID | AB_3671982 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P16885 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PLCG2 (Tyr1217) antibody 84456-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |