Phospho-PLCG2 (Tyr1217) Recombinant antibody
Phospho-PLCG2 (Tyr1217) Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240631F2
验证数据展示
经过测试的应用
Positive WB detected in | pervanadate treated Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy PLCG2 Ready-To-Use IHC Kit. PLCG2 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84456-1-RR targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | phospholipase C, gamma 2 (phosphatidylinositol-specific) |
Synonyms | |
Calculated Molecular Weight | 148 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | BC007565 |
Gene Symbol | PLCG2 |
Gene ID (NCBI) | 5336 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P16885 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Phospho-PLCG2 (Tyr1217) antibody 84456-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |