Phospho-POLR2A (Ser2) Polyclonal antibody

Phospho-POLR2A (Ser2) Polyclonal Antibody for WB, ELISA
Cat No. 30888-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inC2C12 cells, λ phosphatase treated C2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30888-1-AP targets Phospho-POLR2A (Ser2) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Synonyms hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Calculated Molecular Weight 1970 aa, 217 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC137231
Gene Symbol POLR2A
Gene ID (NCBI) 5430
RRIDAB_3669776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)

实验方案

Product Specific Protocols
WB protocol for Phospho-POLR2A (Ser2) antibody 30888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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