验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, λ phosphatase treated C2C12 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30888-1-AP targets Phospho-POLR2A (Ser2) in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide A, 220kDa |
| Synonyms | hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2 |
| Calculated Molecular Weight | 1970 aa, 217 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | BC137231 |
| Gene Symbol | POLR2A |
| Gene ID (NCBI) | 5430 |
| RRID | AB_3669776 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24928 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-POLR2A (Ser2) antibody 30888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |