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验证数据展示

  • WB analysis using 29874-1-AP

    WB analysis using 29874-1-AP

    Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 29874-1-AP (Phospho-PPP1CA (Thr320) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

Phospho-PPP1CA (Thr320) Polyclonal antibody

Phospho-PPP1CA (Thr320) Polyclonal Antibody for WB, ELISA
Cat No. 29874-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, ELISA

别名

Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CA Ready-To-Use IHC Kit. PPP1CA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29874-1-AP targets Phospho-PPP1CA (Thr320) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, alpha isoform
别名 Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA
计算分子量 330 aa, 38 kDa
观测分子量37 kDa
GenBank蛋白编号BC001888
基因名称 PPP1CA
Gene ID (NCBI) 5499
RRIDAB_2935422
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62136
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

实验方案

Product Specific Protocols
WB protocol for Phospho-PPP1CA (Thr320) antibody 29874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice

Authors - Wen Yin
View Article