Phospho-PPP1CA (Thr320) Polyclonal antibody

Phospho-PPP1CA (Thr320) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29874-1-AP

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Synonyms

Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA



经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CA Ready-To-Use IHC Kit. PPP1CA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29874-1-AP targets Phospho-PPP1CA (Thr320) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, alpha isoform
Synonyms Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA
Calculated Molecular Weight 330 aa, 38 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC001888
Gene Symbol PPP1CA
Gene ID (NCBI) 5499
RRIDAB_2935422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62136
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

实验方案

Product Specific Protocols
WB protocol for Phospho-PPP1CA (Thr320) antibody 29874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols