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验证数据展示

  • WB analysis using 29072-1-AP

    WB analysis using 29072-1-AP

    Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • WB analysis using 29072-1-AP

    WB analysis using 29072-1-AP

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Phospho-PRAS40 (Thr246) Polyclonal antibody

Phospho-PRAS40 (Thr246) Polyclonal Antibody for WB, ELISA
Cat No. 29072-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

AKT1S1, AKTS1, Lobe, Phospho-PRAS40 (Thr246), PRAS40, Proline rich AKT1 substrate 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inIGF-1 treated MCF-7 cells, Calyculin A treated PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29072-1-AP targets Phospho-PRAS40 (Thr246) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AKT1 substrate 1 (proline-rich)
别名 AKT1S1, AKTS1, Lobe, Phospho-PRAS40 (Thr246), PRAS40, Proline rich AKT1 substrate 1
计算分子量 27 kDa
观测分子量 40 kDa
GenBank蛋白编号BC051844
基因名称 PRAS40
Gene ID (NCBI) 84335
RRIDAB_2935413
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)

实验方案

Product Specific Protocols
WB protocol for Phospho-PRAS40 (Thr246) antibody 29072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols