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Phospho-PRAS40 (Thr246) Polyclonal antibody

Phospho-PRAS40 (Thr246) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29072-1-AP

Print datasheet

Synonyms

AKT1S1, AKTS1, Lobe, Phospho-PRAS40 (Thr246), PRAS40, Proline rich AKT1 substrate 1

验证数据展示

  • WB analysis using 29072-1-AP

    WB analysis using 29072-1-AP

    Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • WB analysis using 29072-1-AP

    WB analysis using 29072-1-AP

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inIGF-1 treated MCF-7 cells, Calyculin A treated PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29072-1-AP targets Phospho-PRAS40 (Thr246) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name AKT1 substrate 1 (proline-rich)
Synonyms AKT1S1, AKTS1, Lobe, Phospho-PRAS40 (Thr246), PRAS40, Proline rich AKT1 substrate 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC051844
Gene Symbol PRAS40
Gene ID (NCBI) 84335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96B36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)

实验方案

Product Specific Protocols
WB protocol for Phospho-PRAS40 (Thr246) antibody 29072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols