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验证数据展示

  • WB analysis of HeLa using 82309-1-RR

    WB analysis of HeLa using 82309-1-RR

    Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control.

Phospho-RASGRF1 (Ser927) Recombinant antibody

Phospho-RASGRF1 (Ser927) Recombinant Antibody for WB, ELISA
Cat No. 82309-1-RR

产品说明书

CloneNo. 5K3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

p RASGRF1, p RASGRF1 (Ser927), p RASGRF1 Ser927, Phospho RASGRF1, Phospho RASGRF1 (Ser927)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inForskolin treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82309-1-RR targets Phospho-RASGRF1 (Ser927) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Ras protein-specific guanine nucleotide-releasing factor 1
别名 p RASGRF1, p RASGRF1 (Ser927), p RASGRF1 Ser927, Phospho RASGRF1, Phospho RASGRF1 (Ser927)
计算分子量 134 kDa
观测分子量145 kDa
GenBank蛋白编号BC040275
基因名称 RASGRF1
Gene ID (NCBI) 5923
RRIDAB_3085358
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13972
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)

实验方案

Product Specific Protocols
WB protocol for Phospho-RASGRF1 (Ser927) antibody 82309-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols