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验证数据展示

  • WB analysis using 14444-1-AP

    WB analysis using 14444-1-AP

    Non-treated and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 14444-1-AP (Phospho-RCC1 (Ser11) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RCC1 antibody (22142-1-AP) subsequently.

Phospho-RCC1 (Ser11) Polyclonal antibody

Phospho-RCC1 (Ser11) Polyclonal Antibody for WB, ELISA
Cat No. 14444-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Cell cycle regulatory protein, CHC1, Phospho-RCC1 (Ser11), RCC1, RCC1 I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14444-1-AP targets Phospho-RCC1 (Ser11) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of chromosome condensation 1
别名 Cell cycle regulatory protein, CHC1, Phospho-RCC1 (Ser11), RCC1, RCC1 I
计算分子量 48 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_001048194
基因名称 RCC1
Gene ID (NCBI) 1104
RRIDAB_2179583
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18754
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser11) antigen.

实验方案

Product Specific Protocols
WB protocol for Phospho-RCC1 (Ser11) antibody 14444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols