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验证数据展示

  • WB analysis using 29117-1-AP

    WB analysis using 29117-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29117-1-AP (Phospho-RICTOR (Thr1135) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

Phospho-RICTOR (Thr1135) Polyclonal antibody

Phospho-RICTOR (Thr1135) Polyclonal Antibody for WB, ELISA
Cat No. 29117-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

AVO3 homolog, DKFZp686B11164, hAVO3, KIAA1999, mAVO3, Phospho-RICTOR (Thr1135 ), RICTOR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL

¥2480/ 100 μL

浓度: 450 μg/ml

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经过测试的应用

Positive WB detected inPDGF treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RICTOR Ready-To-Use IHC Kit. RICTOR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29117-1-AP targets Phospho-RICTOR (Thr1135) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name rapamycin-insensitive companion of mTOR
Synonyms AVO3 homolog, DKFZp686B11164, hAVO3, KIAA1999, mAVO3, Phospho-RICTOR (Thr1135 ), RICTOR
Calculated Molecular Weight 192 kDa
Observed Molecular Weight192-200 kDa
GenBank Accession NumberBC029608
Gene Symbol RICTOR
Gene ID (NCBI) 253260
RRIDAB_3085343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6R327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rictor is the core component of the mammalian target of rapamycin complex 2 (mTORC2), and loss of this protein leads to an immunodeficiency. RICTOR is regulated by phosphorylation at threonine 1135 (Thr1135) by S6K, downstream of PI3K, which inactivates mTORC2 and therefore inhibits AKT phosphorylation at Ser473. (PMID: 26356562, PMID: 28821013)

实验方案

Product Specific Protocols
WB protocol for Phospho-RICTOR (Thr1135) antibody 29117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols