• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29117-1-AP

    WB analysis using 29117-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29117-1-AP (Phospho-RICTOR (Thr1135) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

Phospho-RICTOR (Thr1135) Polyclonal antibody

Phospho-RICTOR (Thr1135) Polyclonal Antibody for WB, ELISA
Cat No. 29117-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

AVO3 homolog, DKFZp686B11164, hAVO3, KIAA1999, mAVO3, Phospho-RICTOR (Thr1135 ), RICTOR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inPDGF treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RICTOR Ready-To-Use IHC Kit. RICTOR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29117-1-AP targets Phospho-RICTOR (Thr1135) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rapamycin-insensitive companion of mTOR
别名 AVO3 homolog, DKFZp686B11164, hAVO3, KIAA1999, mAVO3, Phospho-RICTOR (Thr1135 ), RICTOR
计算分子量 192 kDa
观测分子量192-200 kDa
GenBank蛋白编号BC029608
基因名称 RICTOR
Gene ID (NCBI) 253260
RRIDAB_3085343
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6R327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rictor is the core component of the mammalian target of rapamycin complex 2 (mTORC2), and loss of this protein leads to an immunodeficiency. RICTOR is regulated by phosphorylation at threonine 1135 (Thr1135) by S6K, downstream of PI3K, which inactivates mTORC2 and therefore inhibits AKT phosphorylation at Ser473. (PMID: 26356562, PMID: 28821013)

实验方案

Product Specific Protocols
WB protocol for Phospho-RICTOR (Thr1135) antibody 29117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols