验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells |
| Positive IF/ICC detected in | λ phosphatase treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
28983-1-AP targets Phospho-RPS6KA1 (Ser380) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
| 别名 | p RPS6KA1, p RPS6KA1 (Ser380), p RPS6KA1 Ser380, Phospho RPS6KA1, Phospho RPS6KA1 (Ser380) |
| 计算分子量 | 735 aa, 83 kDa |
| 观测分子量 | 83-90 kDa |
| GenBank蛋白编号 | BC014966 |
| 基因名称 | RPS6KA1 |
| Gene ID (NCBI) | 6195 |
| RRID | AB_2881237 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15418 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RPS6KA1 (Ser380) antibody 28983-1-AP | Download protocol |
| IF protocol for Phospho-RPS6KA1 (Ser380) antibody 28983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer | ||
Cell Death Dis Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration |