• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80108-1-RR (same clone as 80108-1-PBS)

    WB analysis using 80108-1-RR (same clone as 80108-1-PBS)

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80108-1-RR (same clone as 80108-1-PBS)

    FC experiment of Jurkat using 80108-1-RR (same clone as 80108-1-PBS)

    1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80108-1-RR (same clone as 80108-1-PBS)

    WB analysis of HeLa using 80108-1-RR (same clone as 80108-1-PBS)

    WB result of Phospho-RPS6KA1 (Ser380) antibody (80108-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-RPS6KA1 (Ser380) transfected HeLa cells. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 80108-1-RR (same clone as 80108-1-PBS)

    IHC staining of human malignant melanoma using 80108-1-RR (same clone as 80108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Phospho-RPS6KA1 (Ser380) antibody included
  • IF Staining of HeLa using 80108-1-RR (same clone as 80108-1-PBS)

    IF Staining of HeLa using 80108-1-RR (same clone as 80108-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Phospho-RPS6KA1 (Ser380) antibody (80108-1-RR, Clone: 7F23 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Phospho-RPS6KA1 (Ser380) Recombinant antibody, PBS Only

Phospho-RPS6KA1 (Ser380) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80108-1-PBS

产品说明书

CloneNo. 7F23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.

产品信息

80108-1-PBS targets Phospho-RPS6KA1 (Ser380) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 1
别名 RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1
计算分子量 735 aa, 83 kDa
观测分子量83-90 kDa
GenBank蛋白编号BC014966
基因名称 RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2918865
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15418
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)