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验证数据展示

  • WB analysis using 29225-1-AP

    WB analysis using 29225-1-AP

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

  • WB analysis using 29225-1-AP

    WB analysis using 29225-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of NIH/3T3 using 29225-1-AP

    IF Staining of NIH/3T3 using 29225-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated NIH/3T3 cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (29225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-RPS6KA1 (Thr359/Ser363) Polyclonal antibody

Phospho-RPS6KA1 (Thr359/Ser363) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29225-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, ELISA

别名

p RPS6KA1, p RPS6KA1 (Thr359/Ser363), p RPS6KA1 Thr359/Ser363, Phospho RPS6KA1, Phospho RPS6KA1 (Thr359/Ser363)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated NIH/3T3 cells, PDGF treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29225-1-AP targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 1
别名 p RPS6KA1, p RPS6KA1 (Thr359/Ser363), p RPS6KA1 Thr359/Ser363, Phospho RPS6KA1, Phospho RPS6KA1 (Thr359/Ser363)
计算分子量 735 aa, 83 kDa
观测分子量83-90 kDa
GenBank蛋白编号BC014966
基因名称 RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2918254
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15418
储存缓冲液 PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

实验方案

Product Specific Protocols
WB protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 29225-1-APDownload protocol
IF protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 29225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

JTCVS Open

Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium

Authors - Dwight D Harris
View Article