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Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29223-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

    WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 29223-1-PBS in a different storage buffer formulation.

  • WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

    WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29223-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

产品信息

29223-1-PBS targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S6
Synonyms
Calculated Molecular Weight 29 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2918253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.