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Species Reactivity
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验证数据展示

  • FC experiment of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

    FC experiment of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone: 5J13, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for FC (Intra), Indirect ELISA
Cat No. 81184-1-PBS

产品说明书

CloneNo. 5J13

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), Indirect ELISA

别名

p S6 Ribosomal protein Ser240/244, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser240/244)

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

产品信息

81184-1-PBS targets Phospho-S6 Ribosomal protein (Ser240/244) in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S6
别名 p S6 Ribosomal protein Ser240/244, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser240/244)
观测分子量32 kDa
GenBank蛋白编号NM_001010
基因名称 RPS6
Gene ID (NCBI) 6194
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.