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验证数据展示

  • WB analysis using 31597-1-AP

    WB analysis using 31597-1-AP

    Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 31597-1-AP (Phospho-SAMHD1 (Thr592) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with SAMHD1 (12586-1-AP) antibody as a loading control.

Phospho-SAMHD1 (Thr592) Polyclonal antibody

Phospho-SAMHD1 (Thr592) Polyclonal Antibody for WB, ELISA
Cat No. 31597-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

phospho-SAMHD1, Phospho-SAMHD1-Thr592, phospho-T592-SAMHD1, p-SAMHD1, p-SAMHD1 (Thr592)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, λ phosphatase treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31597-1-AP targets Phospho-SAMHD1 (Thr592) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SAM domain and HD domain 1
别名 phospho-SAMHD1, Phospho-SAMHD1-Thr592, phospho-T592-SAMHD1, p-SAMHD1, p-SAMHD1 (Thr592)
计算分子量 626 aa, 72 kDa
观测分子量72 kDa
GenBank蛋白编号BC036450
基因名称 SAMHD1
Gene ID (NCBI) 25939
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3Z3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAMHD1 is a GTP/dGTP-activated deoxynucleotide triphosphohydrolase. Human SAMHD1 is a 626 amino acid protein comprising an N-terminal nuclear localisation signal, two major structural domains. SAMHD1 has been reported to have alternative nuclease and/or RNA binding activities that, dependent on the cellular circumstances, can also mediate restriction of HIV-1. In addition, restriction is regulated by Threonine 592 phosphorylation and removing this regulation may enable SAMHD1 to inhibit HIV-1 in cycling cells.

实验方案

Product Specific Protocols
WB protocol for Phospho-SAMHD1 (Thr592) antibody 31597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols