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验证数据展示

  • WB analysis using 31597-1-AP

    WB analysis using 31597-1-AP

    Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 31597-1-AP (Phospho-SAMHD1 (Thr592) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with SAMHD1 (12586-1-AP) antibody as a loading control.

Phospho-SAMHD1 (Thr592) Polyclonal antibody

Phospho-SAMHD1 (Thr592) Polyclonal Antibody for WB, ELISA
Cat No. 31597-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

phospho-SAMHD1, Phospho-SAMHD1-Thr592, phospho-T592-SAMHD1, p-SAMHD1, p-SAMHD1 (Thr592)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, λ phosphatase treated THP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31597-1-AP targets Phospho-SAMHD1 (Thr592) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SAM domain and HD domain 1
Synonyms phospho-SAMHD1, Phospho-SAMHD1-Thr592, phospho-T592-SAMHD1, p-SAMHD1, p-SAMHD1 (Thr592)
Calculated Molecular Weight 626 aa, 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC036450
Gene Symbol SAMHD1
Gene ID (NCBI) 25939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3Z3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAMHD1 is a GTP/dGTP-activated deoxynucleotide triphosphohydrolase. Human SAMHD1 is a 626 amino acid protein comprising an N-terminal nuclear localisation signal, two major structural domains. SAMHD1 has been reported to have alternative nuclease and/or RNA binding activities that, dependent on the cellular circumstances, can also mediate restriction of HIV-1. In addition, restriction is regulated by Threonine 592 phosphorylation and removing this regulation may enable SAMHD1 to inhibit HIV-1 in cycling cells.

实验方案

Product Specific Protocols
WB protocol for Phospho-SAMHD1 (Thr592) antibody 31597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols