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Phospho-SMAD1 (Ser187) Polyclonal antibody

Phospho-SMAD1 (Ser187) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28865-1-AP

Print datasheet

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, Phospho-SMAD1 (Ser187), SMAD 1, SMAD family member 1, SMAD1

验证数据展示

  • WB analysis using 28865-1-AP

    WB analysis using 28865-1-AP

    Non-treated HepG2 and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 28865-1-AP (Phospho-SMAD1 (Ser187) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inBMP2 treated HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMAD1 Ready-To-Use IHC Kit. SMAD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28865-1-AP targets Phospho-SMAD1 (Ser187) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityrat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMAD family member 1
Synonyms BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, Phospho-SMAD1 (Ser187), SMAD 1, SMAD family member 1, SMAD1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
RRIDAB_2881221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS).

实验方案

Product Specific Protocols
WB protocol for Phospho-SMAD1 (Ser187) antibody 28865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Comb Chem High Throughput Screen

Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve

Authors - Wanjing Li
View Article
ratWB

Cardiol Res Pract

Atorvastatin Inhibits Ferroptosis of H9C2 Cells by regulatingSMAD7/Hepcidin Expression to Improve Ischemia-Reperfusion Injury

Authors - You Peng
View Article