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Phospho-SMAD1 (Ser214) Polyclonal antibody, PBS Only

Phospho-SMAD1 (Ser214) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28895-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28895-1-AP (same clone as 28895-1-PBS)

    WB analysis using 28895-1-AP (same clone as 28895-1-PBS)

    Non-treated HepG2 and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 28895-1-AP (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 28895-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMAD1 Ready-To-Use IHC Kit. SMAD1 primary antibody included.

产品信息

28895-1-PBS targets Phospho-SMAD1 (Ser214) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMAD family member 1
Synonyms
Calculated Molecular Weight 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
RRIDAB_2918212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15797
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)