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验证数据展示

  • WB analysis of A431 using 81433-1-RR (same clone as 81433-1-PBS)

    WB analysis of A431 using 81433-1-RR (same clone as 81433-1-PBS)

    Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81433-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81433-1-RR (same clone as 81433-1-PBS)

    WB analysis of HepG2 using 81433-1-RR (same clone as 81433-1-PBS)

    Non-treated and Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81433-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 81433-1-RR (same clone as 81433-1-PBS)

    WB analysis of NIH/3T3 using 81433-1-RR (same clone as 81433-1-PBS)

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81433-1-PBS in a different storage buffer formulation.

Phospho-SMAD1 (Ser214) Recombinant antibody, PBS Only

Phospho-SMAD1 (Ser214) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81433-1-PBS

产品说明书

CloneNo. 3K8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

SMAD1, JV4-1, JV41, hSMAD1, BSP-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMAD1 Ready-To-Use IHC Kit. SMAD1 primary antibody included.

产品信息

81433-1-PBS targets Phospho-SMAD1 (Ser214) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 1
别名 SMAD1, JV4-1, JV41, hSMAD1, BSP-1
计算分子量 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC001878
基因名称 SMAD1
Gene ID (NCBI) 4086
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15797
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)