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验证数据展示

  • WB analysis using 80144-2-RR

    WB analysis using 80144-2-RR

    Non-treated and BMP2 (HZ-1128) treated HeLa cells were subjected to SDS PAGE followed by western blot with 80144-2-RR (Phospho-SMAD9 (Ser465/467) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-SMAD9 (Ser465/467) Recombinant antibody

Phospho-SMAD9 (Ser465/467) Recombinant Antibody for WB, ELISA
Cat No. 80144-2-RR

产品说明书

CloneNo. 240470A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

P SMAD5 (Ser463/465), p SMAD9, Phos SMAD1 (Ser463/465), Phos SMAD9, Phospho SMAD9 (Ser465/467)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBMP2 treated HeLa cells

The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463/465.

Planning an IHC experiment? We recommend our IHCeasy SMAD9 Ready-To-Use IHC Kit. SMAD9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80144-2-RR targets Phospho-SMAD9 (Ser465/467) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 9
别名 P SMAD5 (Ser463/465), p SMAD9, Phos SMAD1 (Ser463/465), Phos SMAD9, Phospho SMAD9 (Ser465/467)
计算分子量 430 aa, 49 kDa
观测分子量 55 kDa
GenBank蛋白编号BC011559
基因名称 SMAD9
Gene ID (NCBI) 4093
RRIDAB_3670469
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15198
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein. The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463/465.

实验方案

Product Specific Protocols
WB protocol for Phospho-SMAD9 (Ser465/467) antibody 80144-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols