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验证数据展示

  • WB analysis using 80144-2-RR

    WB analysis using 80144-2-RR

    Non-treated and BMP2 (HZ-1128) treated HeLa cells were subjected to SDS PAGE followed by western blot with 80144-2-RR (Phospho-SMAD9 (Ser465/467) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-SMAD9 (Ser465/467) Recombinant antibody

Phospho-SMAD9 (Ser465/467) Recombinant Antibody for WB, ELISA
Cat No. 80144-2-RR
CloneNo. 240470A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

P SMAD5 (Ser463/465), p SMAD9, Phos SMAD1 (Ser463/465), Phos SMAD9, Phospho SMAD9 (Ser465/467)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inBMP2 treated HeLa cells

The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463/465.

Planning an IHC experiment? We recommend our IHCeasy SMAD9 Ready-To-Use IHC Kit. SMAD9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80144-2-RR targets Phospho-SMAD9 (Ser465/467) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 9
Synonyms P SMAD5 (Ser463/465), p SMAD9, Phos SMAD1 (Ser463/465), Phos SMAD9, Phospho SMAD9 (Ser465/467)
Calculated Molecular Weight 430 aa, 49 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC011559
Gene Symbol SMAD9
Gene ID (NCBI) 4093
RRIDAB_3670469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein. The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463/465.

实验方案

Product Specific Protocols
WB protocol for Phospho-SMAD9 (Ser465/467) antibody 80144-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols