验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19561-1-AP targets Phospho-SPHK1 (Ser225) in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sphingosine kinase 1 |
| 别名 | Phospho-SPHK1 (Ser225), SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPHK1-Phospho-Ser225, SPK, SPK 1 |
| 计算分子量 | 51 kDa |
| 观测分子量 | 45-47 kDa |
| GenBank蛋白编号 | NM_182965 |
| 基因名称 | SPHK1 |
| Gene ID (NCBI) | 8877 |
| RRID | AB_10950399 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-SPHK1 (Ser225) antibody 19561-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation. | ||
Ecotoxicol Environ Saf A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway. | ||
Biochim Biophys Acta Mol Basis Dis Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy. | ||
Phytomedicine Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation. | ||
Phytother Res Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro. |
