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Phospho-STAT1 (Ser727) Recombinant antibody

Phospho-STAT1 (Ser727) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240897D10

Cat no : 80683-7-RR

Print datasheet

Synonyms

phospho-S727 STAT1, Phospho STAT1 (Ser727), Phospho STAT1, p Stat1 (S727), phospho-STAT1

验证数据展示

  • WB analysis using 80683-7-RR

    WB analysis using 80683-7-RR

    Non treated and IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 80683-7-RR (Phospho-STAT1 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inIFN gamma treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80683-7-RR targets Phospho-STAT1 (Ser727) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms phospho-S727 STAT1, Phospho STAT1 (Ser727), Phospho STAT1, p Stat1 (S727), phospho-STAT1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight84-91 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant duringlipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT1 (Ser727) antibody 80683-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols