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Phospho-STAT1 (Tyr701) Polyclonal antibody

Phospho-STAT1 (Tyr701) Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 28979-1-AP

Print datasheet

Synonyms

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

验证数据展示

  • WB analysis using 28979-1-AP

    WB analysis using 28979-1-AP

    Non-treated THP-1 cells, IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28979-1-AP (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28979-1-AP

    IF Staining of HeLa using 28979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using Phospho-STAT1 (Tyr701) antibody (28979-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of THP-1 using 28979-1-AP

    FC experiment of THP-1 using 28979-1-AP

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.25 ug Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inIFN gamma and LPS treated THP-1 cells
Positive IF/ICC detected inIFN alpha treated HeLa cells
Positive FC (Intra) detected inIFN gamma treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28979-1-AP targets Phospho-STAT1 (Tyr701) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3
Calculated Molecular Weight 83 kDa
Observed Molecular Weight84-91 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-APDownload protocol
IF protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Transl Med

Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils

Authors - Ye Xie
View Article