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Phospho-STAT1 (Tyr701) Polyclonal antibody, PBS Only

Phospho-STAT1 (Tyr701) Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 28979-1-PBS

Print datasheet

Synonyms

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

验证数据展示

  • WB analysis using 28979-1-AP (same clone as 28979-1-PBS)

    WB analysis using 28979-1-AP (same clone as 28979-1-PBS)

    Non-treated THP-1 cells, IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28979-1-AP (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28979-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28979-1-AP (same clone as 28979-1-PBS)

    IF Staining of HeLa using 28979-1-AP (same clone as 28979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using Phospho-STAT1 (Tyr701) antibody (28979-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28979-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 28979-1-AP (same clone as 28979-1-PBS)

    FC experiment of THP-1 using 28979-1-AP (same clone as 28979-1-PBS)

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.25 ug Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 28979-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

产品信息

28979-1-PBS targets Phospho-STAT1 (Tyr701) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3
Calculated Molecular Weight 83 kDa
Observed Molecular Weight84-91 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42224
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).