Phospho-STAT3 (Ser727) Polyclonal antibody

Phospho-STAT3 (Ser727) Polyclonal Antibody for WB, ELISA
Cat No. 28945-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, ELISA

p-STAT3, Phospho-STAT3, p STAT3 (Ser727), p-STAT3 (Ser727), STAT3 (Ser727)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 11 publications below

产品信息

28945-1-AP targets Phospho-STAT3 (Ser727) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal transducer and activator of transcription 3 (acute-phase response factor)
别名 p-STAT3, Phospho-STAT3, p STAT3 (Ser727), p-STAT3 (Ser727), STAT3 (Ser727)
计算分子量 770 aa, 88 kDa
观测分子量92-100 kDa
GenBank蛋白编号BC000627
基因名称 STAT3
Gene ID (NCBI) 6774
RRIDAB_3206262
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP40763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 28945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

The malic acid inhibiting inflammation in ankylosing spondylitis by interfering M1 macrophage polarization

Authors - Zhou Ji
humanWB

Biofactors

α-Asarone alleviates allergic asthma by stabilizing mast cells through inhibition of ERK/JAK2-STAT3 pathway

Authors - Haoyun Bai
mouse,ratWB

J Ginseng Res

Ginsenoside Rg1 alleviates vascular remodeling in hypoxia-induced pulmonary hypertension mice through the calpain-1/STAT3 signaling pathway

Authors - Chenyang Ran
human,mouseWB

J Ethnopharmacol

Cyberpharmacology uncover the mechanism of the total Rhizoma Coptidis extracts ameliorate chronic atrophic gastritis

Authors - Zhengcai Ma
human,ratWB

Adv Sci (Weinh)

H2 S-Releasing Versatile Montmorillonite Nanoformulation Trilogically Renovates the Gut Microenvironment for Inflammatory Bowel Disease Modulation

Authors - Ting Jin
humanWB

Int J Biol Macromol

A novel angiotensin I-converting enzyme inhibitory peptide APPLRP from Grifola frondosa ameliorated the Ang II-induced vascular modeling in zebrafish model by mediating smooth muscle cells

Authors - Tianyuan Song
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