验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, λ phosphatase treated HEK-293T cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 11 publications below |
产品信息
28945-1-AP targets Phospho-STAT3 (Ser727) in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | signal transducer and activator of transcription 3 (acute-phase response factor) |
别名 | p-STAT3, Phospho-STAT3, p STAT3 (Ser727), p-STAT3 (Ser727), STAT3 (Ser727) |
计算分子量 | 770 aa, 88 kDa |
观测分子量 | 92-100 kDa |
GenBank蛋白编号 | BC000627 |
基因名称 | STAT3 |
Gene ID (NCBI) | 6774 |
RRID | AB_3206262 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P40763 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-STAT3 (Ser727) antibody 28945-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int Immunopharmacol The malic acid inhibiting inflammation in ankylosing spondylitis by interfering M1 macrophage polarization | ||
Biofactors α-Asarone alleviates allergic asthma by stabilizing mast cells through inhibition of ERK/JAK2-STAT3 pathway | ||
J Ginseng Res Ginsenoside Rg1 alleviates vascular remodeling in hypoxia-induced pulmonary hypertension mice through the calpain-1/STAT3 signaling pathway | ||
J Ethnopharmacol Cyberpharmacology uncover the mechanism of the total Rhizoma Coptidis extracts ameliorate chronic atrophic gastritis | ||
Adv Sci (Weinh) H2 S-Releasing Versatile Montmorillonite Nanoformulation Trilogically Renovates the Gut Microenvironment for Inflammatory Bowel Disease Modulation | ||
Int J Biol Macromol A novel angiotensin I-converting enzyme inhibitory peptide APPLRP from Grifola frondosa ameliorated the Ang II-induced vascular modeling in zebrafish model by mediating smooth muscle cells |