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Phospho-STAT3 (Ser727) Polyclonal antibody

Phospho-STAT3 (Ser727) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28945-1-AP

Print datasheet

Synonyms

Acute phase response factor, APRF, FLJ20882, HIES, Phospho-STAT3 (Ser727), STAT3

验证数据展示

  • WB analysis using 28945-1-AP

    WB analysis using 28945-1-AP

    Phosphatase inhibitor treated HEK-293T cells, λ phosphatase treated HEK-293T cells, and non-treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28945-1-AP (Phospho-STAT3 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28945-1-AP targets Phospho-STAT3 (Ser727) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Synonyms Acute phase response factor, APRF, FLJ20882, HIES, Phospho-STAT3 (Ser727), STAT3
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight92-100 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 28945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biofactors

α-Asarone alleviates allergic asthma by stabilizing mast cells through inhibition of ERK/JAK2-STAT3 pathway

Authors - Haoyun Bai
View Article