验证数据展示
经过测试的应用
| Positive WB detected in | GM-CSF treated TF-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
28951-1-AP targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal transducer and activator of transcription 5A |
| 别名 | Phospho-STAT5A(Ser726)/STAT5B (Ser731), STAT5A, STAT5, MGF |
| 计算分子量 | 794 aa, 92 kDa |
| 观测分子量 | 90-95 kDa |
| GenBank蛋白编号 | BC027036 |
| 基因名称 | STAT5A |
| Gene ID (NCBI) | 6776 |
| RRID | AB_2918220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42229 |
| 储存缓冲液 | PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. This antibody can recognize the phosphorylation of STAT5A at Ser726 and the phosphorylation of STAT5B at Ser731.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody 28951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Endocrinology A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration | ||
PeerJ RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA |