Phospho-STAT5A (Ser726)/STAT5B (Ser731) Polyclonal antibody

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28951-1-AP

Print datasheet

Synonyms

Phospho-STAT5A(Ser726)/STAT5B (Ser731), STAT5A, STAT5, MGF



经过测试的应用

Positive WB detected inGM-CSF treated TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28951-1-AP targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 5A
Synonyms Phospho-STAT5A(Ser726)/STAT5B (Ser731), STAT5A, STAT5, MGF
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2918220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42229
Storage Buffer PBS with 0.1% sodium azide, 50% glycerol pH 7.3 and 0.05%BSA.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. This antibody can recognize the phosphorylation of STAT5A at Ser726 and the phosphorylation of STAT5B at Ser731.

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody 28951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Endocrinology

A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration

Authors - Ruonan Li
humanWB

PeerJ

RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA

Authors - Jin Fan