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验证数据展示

  • WB analysis using 28949-1-AP

    WB analysis using 28949-1-AP

    Non-treated TF-1 and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 28949-1-AP (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • FC experiment of TF-1 using 28949-1-AP

    FC experiment of TF-1 using 28949-1-AP

    1x10^6 GM-CSF treated TF-1 cells were intracellularly stained with 0.13 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal antibody (28949-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal antibody

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 28949-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

STAT5A, STAT5, MGF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inGM-CSF treated TF-1 cells
Positive FC (Intra) detected inGM-CSF treated TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28949-1-AP targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal transducer and activator of transcription 5A
别名 STAT5A, STAT5, MGF
计算分子量 794 aa, 92 kDa
观测分子量92-100 kDa
GenBank蛋白编号BC027036
基因名称 STAT5A
Gene ID (NCBI) 6776
RRIDAB_2918219
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42229
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody 28949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols