Phospho-STK11/LKB1 (Thr189) Polyclonal antibody
Phospho-STK11/LKB1 (Thr189) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated PC-3 cells, UV treated NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28965-1-AP targets Phospho-STK11/LKB1 (Thr189) in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | serine/threonine kinase 11 |
| Synonyms | LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC007981 |
| Gene Symbol | LKB1 |
| Gene ID (NCBI) | 6794 |
| RRID | AB_2918225 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15831 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-STK11/LKB1 (Thr189) antibody 28965-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |