验证数据展示
经过测试的应用
Positive WB detected in | Staurosporine treated Ramos cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
产品信息
28953-1-AP targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | serine/threonine kinase 4 |
别名 | DKFZp686A2068, KRS2, MST 1, MST1, MST1 (T183)/MST2(T180), MST2, Phospho-MST1 (T183)/MST2(T180), Phospho-MST1 (Thr183)/MST2 (Thr180), serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3 |
计算分子量 | 56 kDa |
观测分子量 | 52-56 kDa |
GenBank蛋白编号 | BC005231 |
基因名称 | MST1 |
Gene ID (NCBI) | 6789 |
RRID | AB_2918221 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13043 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1/2 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1/2 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1/2 because phosphorylation of Thr183/180 causes the cleavage of MST1 by caspases under apoptotic conditions.
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-MST1 (Thr183)/MST2 (Thr180) antibody 28953-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Biol Sci S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development | ||
bioRxiv The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells | ||
Discov Oncol Upregulated GPRC5A disrupting the Hippo pathway promotes the proliferation and migration of pancreatic cancer cells via the cAMP-CREB axis | ||
Cell Death Dis PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling | ||
J Cell Physiol m6 A-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma | ||