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验证数据展示

  • WB analysis using 30895-1-AP

    WB analysis using 30895-1-AP

    Phosphatase inhibitor treated HEK-293T cells, λ phosphatase treated HEK-293T cells, and non-treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 30895-1-AP (Phospho-TAK1 (Ser439) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-TAK1 (Ser439) Polyclonal antibody

Phospho-TAK1 (Ser439) Polyclonal Antibody for WB, ELISA
Cat No. 30895-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

p TAK1, p TAK1 (Ser439), p TAK1 Ser439, Phospho TAK1, Phospho TAK1 (Ser439)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells

This antibody is equivalent to Ser412 in mice.

Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30895-1-AP targets Phospho-TAK1 (Ser439) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7
Synonyms p TAK1, p TAK1 (Ser439), p TAK1 Ser439, Phospho TAK1, Phospho TAK1 (Ser439)
Calculated Molecular Weight 579 aa, 64 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
RRIDAB_3669777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. TAK1 has been identified as a crucial regulatory component of MAPK and nuclear factor-κB (NF-κB) signaling pathways, which play a key role in lipid metabolism and inflammation. Activated TAK1 then phosphorylates downstream substrates to spark the NF-κB and MAPK signaling pathways. Hence, phospho-TAK1 confers most of its regulatory functions, and inhibiting the hyperactivation of TAK1 should be a promising NASH(Nonalcoholic steatohepatitis ) therapy. (PMID: 34146477) This antibody is equivalent to Ser412 in mice.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAK1 (Ser439) antibody 30895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols