验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, λ phosphatase treated HEK-293T cells |
This antibody is equivalent to Ser412 in mice.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30895-1-AP targets Phospho-TAK1 (Ser439) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase kinase 7 |
| 别名 | p TAK1, p TAK1 (Ser439), p TAK1 Ser439, Phospho TAK1, Phospho TAK1 (Ser439) |
| 计算分子量 | 579 aa, 64 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC017715 |
| 基因名称 | TAK1 |
| Gene ID (NCBI) | 6885 |
| RRID | AB_3669777 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. TAK1 has been identified as a crucial regulatory component of MAPK and nuclear factor-κB (NF-κB) signaling pathways, which play a key role in lipid metabolism and inflammation. Activated TAK1 then phosphorylates downstream substrates to spark the NF-κB and MAPK signaling pathways. Hence, phospho-TAK1 confers most of its regulatory functions, and inhibiting the hyperactivation of TAK1 should be a promising NASH(Nonalcoholic steatohepatitis ) therapy. (PMID: 34146477) This antibody is equivalent to Ser412 in mice.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TAK1 (Ser439) antibody 30895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |