• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of NIH/3T3 using 81363-2-RR

    WB analysis of NIH/3T3 using 81363-2-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • WB analysis of HeLa using 81363-2-RR

    WB analysis of HeLa using 81363-2-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

Phospho-TBC1D1 (Ser660) Recombinant antibody

Phospho-TBC1D1 (Ser660) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81363-2-RR

产品说明书

CloneNo. 7N19

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

TBC1D1, TBC1 domain family member 1, TBC1, KIAA1108, 7N19

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81363-2-RR targets Phospho-TBC1D1 (Ser660) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
别名 TBC1D1, TBC1 domain family member 1, TBC1, KIAA1108, 7N19
计算分子量 133 kDa
观测分子量 160 kDa
GenBank蛋白编号BC050321
基因名称 TBC1D1
Gene ID (NCBI) 23216
RRIDAB_3085355
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86TI0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)

实验方案

Product Specific Protocols
WB protocol for Phospho-TBC1D1 (Ser660) antibody 81363-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols