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验证数据展示

  • WB analysis using 29000-1-AP

    WB analysis using 29000-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

  • WB analysis using 29000-1-AP

    WB analysis using 29000-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

Phospho-TSC2 (Thr1462) Polyclonal antibody

Phospho-TSC2 (Thr1462) Polyclonal Antibody for WB, ELISA
Cat No. 29000-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (1)

应用

WB, ELISA

别名

FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPDGF treated PC-3 cells, PDGF treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29000-1-AP targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tuberous sclerosis 2
别名 FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein
计算分子量 1807 aa, 201 kDa
观测分子量200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
RRIDAB_2918227
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49815
储存缓冲液 PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)

实验方案

Product Specific Protocols
WB protocol for Phospho-TSC2 (Thr1462) antibody 29000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Research (Wash D C)

The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer

Authors - Yue Zhao
View Article