验证数据展示
经过测试的应用
Positive WB detected in | λ phosphatase treated NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30005-1-AP targets Phospho-ULK1 (Ser467) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, Mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | unc-51-like kinase 1 (C. elegans) |
别名 | ATG1, FLJ38455, FLJ46475, KIAA0722, Phospho-ULK1 (Ser467), ULK1, UNC51, Unc51.1 |
观测分子量 | 113-130 kDa |
GenBank蛋白编号 | NM_003565 |
基因名称 | ULK1 |
Gene ID (NCBI) | 8408 |
RRID | AB_3085348 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O75385 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. AMPK increases ULK1 activity by directly phosphorylating Ser467, Ser555, Thr574, and Ser637 at least four sites, which increases the recruitment of autophagy-relevant proteins (ATG proteins) to the membrane domains which affects autophagy at the initiation stage. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-ULK1 (Ser467) antibody 30005-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |