Phospho-ULK1 (Ser467) Polyclonal antibody

Phospho-ULK1 (Ser467) Polyclonal Antibody for WB, ELISA
Cat No. 30005-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, ELISA

ATG1, FLJ38455, FLJ46475, KIAA0722, Phospho-ULK1 (Ser467), ULK1, UNC51, Unc51.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inλ phosphatase treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30005-1-AP targets Phospho-ULK1 (Ser467) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 ATG1, FLJ38455, FLJ46475, KIAA0722, Phospho-ULK1 (Ser467), ULK1, UNC51, Unc51.1
观测分子量113-130 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
RRIDAB_3085348
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75385
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. AMPK increases ULK1 activity by directly phosphorylating Ser467, Ser555, Thr574, and Ser637 at least four sites, which increases the recruitment of autophagy-relevant proteins (ATG proteins) to the membrane domains which affects autophagy at the initiation stage. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367)

实验方案

Product Specific Protocols
WB protocol for Phospho-ULK1 (Ser467) antibody 30005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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