Phospho-ULK1 (Ser467) Polyclonal antibody

Phospho-ULK1 (Ser467) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30005-1-AP

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Synonyms

ATG1, FLJ38455, FLJ46475, KIAA0722, Phospho-ULK1 (Ser467), ULK1, UNC51, Unc51.1



经过测试的应用

Positive WB detected inλ phosphatase treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30005-1-AP targets Phospho-ULK1 (Ser467) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-51-like kinase 1 (C. elegans)
Synonyms ATG1, FLJ38455, FLJ46475, KIAA0722, Phospho-ULK1 (Ser467), ULK1, UNC51, Unc51.1
Observed Molecular Weight113-130 kDa
GenBank Accession NumberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75385
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. AMPK increases ULK1 activity by directly phosphorylating Ser467, Ser555, Thr574, and Ser637 at least four sites, which increases the recruitment of autophagy-relevant proteins (ATG proteins) to the membrane domains which affects autophagy at the initiation stage. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367)

实验方案

Product Specific Protocols
WB protocol for Phospho-ULK1 (Ser467) antibody 30005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols