验证数据展示
经过测试的应用
| Positive WB detected in | λ phosphatase treated HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
29006-1-AP targets Phospho-ULK1 (Thr468) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | unc-51-like kinase 1 (C. elegans) |
| Synonyms | ULK1, Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1 |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_003565 |
| Gene Symbol | ULK1 |
| Gene ID (NCBI) | 8408 |
| RRID | AB_2918229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75385 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ULK1 (Thr468) antibody 29006-1-AP | Download protocol |
| IF protocol for Phospho-ULK1 (Thr468) antibody 29006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Res Bull N-linoleyltyrosine protects neurons against Aβ1-40-induced cell toxicity via autophagy involving the CB2/AMPK/mTOR/ULK1 pathway | ||
Anal Cell Pathol (Amst) Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis | ||
Environ Toxicol Lysine specific demethylase 1 inhibits sodium arsenite activation of HSCs by regulating SESN2/AMPK/ULK1 signaling pathway activity |