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Phospho-XIAP (Ser87) Polyclonal antibody

Phospho-XIAP (Ser87) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28791-1-AP

Print datasheet

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, Phospho-XIAP (Ser87), S87, Ser87, X linked IAP, XIAP, XIAP (Ser87), XIAP S87, XIAP Ser87, XLP2

验证数据展示

  • WB analysis using 28791-1-AP

    WB analysis using 28791-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28791-1-AP (Phospho-XIAP (Ser87) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPDGF treated NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28791-1-AP targets Phospho-XIAP (Ser87) in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name X-linked inhibitor of apoptosis
Synonyms API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, Phospho-XIAP (Ser87), S87, Ser87, X linked IAP, XIAP, XIAP (Ser87), XIAP S87, XIAP Ser87, XLP2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
RRIDAB_2881213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98170
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. XIAP is directly phosphorylated by PKC and that XIAP phosphorylated at Ser87 by PKC is resistant to degradation, protecting cells from apoptosis-inducing stress.

实验方案

Product Specific Protocols
WB protocol for Phospho-XIAP (Ser87) antibody 28791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols