Phospho-YAP1 (Ser397) Polyclonal antibody
Phospho-YAP1 (Ser397) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (2)
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy YAP1 Ready-To-Use IHC Kit. YAP1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29018-1-AP targets Phospho-YAP1 (Ser397) in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | Human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Yes-associated protein 1, 65kDa |
Synonyms | 65 kDa Yes associated protein, Phospho-YAP1 (Ser397), YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog |
Calculated Molecular Weight | 504 aa, 54 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC038235 |
Gene Symbol | YAP1 |
Gene ID (NCBI) | 10413 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P46937 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-YAP1 (Ser397) antibody 29018-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective | ||
Int Immunopharmacol ASIC1a regulates ferroptosis in hepatic stellate cells via the Hippo/Yap-1 pathway in liver fibrosis | ||
Sci Rep Gingival proteomics reveals the role of TGF beta and YAP/TAZ signaling in Raine syndrome fibrosis | ||
Mol Cell Biochem RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma | ||
Arch Biochem Biophys Oridonin suppresses the growth of glioblastoma cells via inhibiting Hippo/YAP axis | ||
J Nat Med Ruscogenin ameliorates dasatinib-induced intestinal barrier dysfunction via ErbB4/YAP and ROCK/MLC pathways |