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Phospho-c-MYC (Ser62) Polyclonal antibody

Phospho-c-MYC (Ser62) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28915-1-AP

Print datasheet

Synonyms

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Phopho-Ser62-MYC, Proto oncogene c Myc, Transcription factor p64

验证数据展示

  • WB analysis using 28915-1-AP

    WB analysis using 28915-1-AP

    Non-treated and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28915-1-AP (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inMG132 treated HEK-293T cells, HEK-293T cells

This site is also equivalent to Ser77 for c-MYC isoform 2 (P01106-2).

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28915-1-AP targets Phospho-c-MYC (Ser62) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Synonyms bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Phopho-Ser62-MYC, Proto oncogene c Myc, Transcription factor p64
Calculated Molecular Weight 49 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)

实验方案

Product Specific Protocols
WB protocol for Phospho-c-MYC (Ser62) antibody 28915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols