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验证数据展示

  • WB analysis using 28915-1-AP

    WB analysis using 28915-1-AP

    Non-treated and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28915-1-AP (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-c-MYC (Ser62) Polyclonal antibody

Phospho-c-MYC (Ser62) Polyclonal Antibody for WB, ELISA
Cat No. 28915-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Phopho-Ser62-MYC, Proto oncogene c Myc, Transcription factor p64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMG132 treated HEK-293T cells, HEK-293T cells

This site is also equivalent to Ser77 for c-MYC isoform 2 (P01106-2).

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28915-1-AP targets Phospho-c-MYC (Ser62) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Phopho-Ser62-MYC, Proto oncogene c Myc, Transcription factor p64
计算分子量 49 kDa
观测分子量55 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_3085340
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)

实验方案

Product Specific Protocols
WB protocol for Phospho-c-MYC (Ser62) antibody 28915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols