• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29032-1-AP

    WB analysis using 29032-1-AP

    Non-treated and λ phosphatase treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29032-1-AP (Phospho-eEF2K (Ser366) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Phospho-eEF2K (Ser366) Polyclonal antibody

Phospho-eEF2K (Ser366) Polyclonal Antibody for WB, ELISA
Cat No. 29032-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

eEF 2 kinase, eEF 2K, eEF2K, HSU93850, Phospho-eEF2K (Ser366)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, λ phosphatase treated Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29032-1-AP targets Phospho-eEF2K (Ser366) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic elongation factor-2 kinase
别名 eEF 2 kinase, eEF 2K, eEF2K, HSU93850, Phospho-eEF2K (Ser366)
计算分子量 725 aa, 100 kDa
观测分子量100 kDa
GenBank蛋白编号BC032665
基因名称 EEF2K
Gene ID (NCBI) 29904
RRIDAB_2918231
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00418
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa.

实验方案

Product Specific Protocols
WB protocol for Phospho-eEF2K (Ser366) antibody 29032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols