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验证数据展示

  • WB analysis using 30811-1-AP

    WB analysis using 30811-1-AP

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 30811-1-AP (Phospho-eEF2K (Thr57) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-eEF2K (Thr57) Polyclonal antibody

Phospho-eEF2K (Thr57) Polyclonal Antibody for WB, ELISA
Cat No. 30811-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, ELISA

别名

eEF 2 kinase, eEF 2K, EEF2K, HSU93850, Phospho-eEF2K (Thr57)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, λ phosphatase treated HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30811-1-AP targets Phospho-eEF2K (Thr57) in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic elongation factor-2 kinase
别名 eEF 2 kinase, eEF 2K, EEF2K, HSU93850, Phospho-eEF2K (Thr57)
计算分子量 725 aa, 100 kDa
观测分子量100 kDa
GenBank蛋白编号BC032665
基因名称 EEF2K
Gene ID (NCBI) 29904
RRIDAB_3669761
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00418
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic elongation factor‐2 kinase (eEF2K), well‐known to be a Ca2+ calmodulin (CaM)‐dependent kinase, is overexpressed in several types of malignancies, most notably Triple negative breast cancer (TNBC). Increasing evidence indicates that eEF2K may modulate the expression of some apoptotic proteins such as XIAP, c‐FLIPL, Bcl‐XL, PI3KCI and p70S6K to inhibit apoptotic process in cancer. On the other hand, it may regulate autophagy involved in AMPK, mTORC1 and ULK1, thereby promoting cancer cell survival. (PMID: 29094413)

实验方案

Product Specific Protocols
WB protocol for Phospho-eEF2K (Thr57) antibody 30811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols