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Phospho-eEF2K (Thr57) Polyclonal antibody

Phospho-eEF2K (Thr57) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30811-1-AP

Print datasheet

Synonyms

eEF 2 kinase, eEF 2K, EEF2K, HSU93850, Phospho-eEF2K (Thr57)

验证数据展示

  • WB analysis using 30811-1-AP

    WB analysis using 30811-1-AP

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 30811-1-AP (Phospho-eEF2K (Thr57) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, λ phosphatase treated HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30811-1-AP targets Phospho-eEF2K (Thr57) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic elongation factor-2 kinase
Synonyms eEF 2 kinase, eEF 2K, EEF2K, HSU93850, Phospho-eEF2K (Thr57)
Calculated Molecular Weight 725 aa, 100 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC032665
Gene Symbol EEF2K
Gene ID (NCBI) 29904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic elongation factor‐2 kinase (eEF2K), well‐known to be a Ca2+ calmodulin (CaM)‐dependent kinase, is overexpressed in several types of malignancies, most notably Triple negative breast cancer (TNBC). Increasing evidence indicates that eEF2K may modulate the expression of some apoptotic proteins such as XIAP, c‐FLIPL, Bcl‐XL, PI3KCI and p70S6K to inhibit apoptotic process in cancer. On the other hand, it may regulate autophagy involved in AMPK, mTORC1 and ULK1, thereby promoting cancer cell survival. (PMID: 29094413)

实验方案

Product Specific Protocols
WB protocol for Phospho-eEF2K (Thr57) antibody 30811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols