Phospho-mTOR (Ser2481) Polyclonal antibody

Phospho-mTOR (Ser2481) Polyclonal Antibody for WB, ELISA
Cat No. 28879-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Mouse and More (3)

应用

WB, ELISA

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inInsulin treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 9 publications below

产品信息

28879-1-AP targets Phospho-mTOR (Ser2481) in WB, ELISA applications and shows reactivity with Mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Mouse
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 12-rapamycin associated protein 1
别名 FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1
计算分子量 289 kDa
观测分子量250-289 kDa
GenBank蛋白编号BC117166
基因名称 mTOR
Gene ID (NCBI) 2475
RRIDAB_3085338
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42345
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTOR, also named FRAP1, FRAP, FRAP2, and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is the kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. mTORC2 is also activated by growth factors but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

实验方案

Product Specific Protocols
WB protocol for Phospho-mTOR (Ser2481) antibody 28879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Anal Cell Pathol (Amst)

Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis

Authors - Junli Zhang
ratWB

Am J Transl Res

Integrating network pharmacology with experimental validation to investigate the mechanism of Wuwei Zishen formula in improving perimenopausal syndrome

Authors - Xuewen Fu
humanWB

Naunyn Schmiedebergs Arch Pharmacol

Network pharmacology-based strategy to reveal the mechanism of pinocembrin against ovarian cancer

Authors - Guanghui Wang
humanWB

Sci Rep

Morphine promotes non-small cell lung cancer progression by downregulating E-cadherin via the PI3K/AKT/mTOR pathway

Authors - Fulei Gu
mouseWB

J Ethnopharmacol

Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling

Authors - Baomei Xia
mouseWB

Chem Biol Interact

Oral administration of sophoricoside (SOP) inhibits neuronal damage and neuroinflammation to curb neurodegeneration in Parkinson's disease

Authors - Hefei Wang
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