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验证数据展示

  • WB analysis using 30887-1-AP

    WB analysis using 30887-1-AP

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 30887-1-AP (Phospho-p130Cas / BCAR1 (Tyr410) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with p130Cas / BCAR1 antibody (16815-1-AP) subsequently.

Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal antibody

Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal Antibody for WB, ELISA
Cat No. 30887-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpervanadate treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30887-1-AP targets Phospho-p130Cas / BCAR1 (Tyr410) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 breast cancer anti-estrogen resistance 1
别名 BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
计算分子量 870 aa, 93 kDa
观测分子量130 kDa
GenBank蛋白编号BC062556
基因名称 BCAR1
Gene ID (NCBI) 9564
RRIDAB_3669775
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p130Cas, encoded by the breast cancer anti-estrogen resistance 1 (BCAR1) gene, is a scaffold protein that integrates large multi-protein complexes in response to stimuli such as hormones, growth factors, and integrin engagement. Upon environmental signals, Src family members and focal adhesion kinase (FAK) phosphorylate the p130Cas substrate domain (SD). This active p130Cas can recruit adaptor proteins such as Crk and Nck to its phosphorylated SD. (PMID: 34830244)

实验方案

Product Specific Protocols
WB protocol for Phospho-p130Cas / BCAR1 (Tyr410) antibody 30887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols