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Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal antibody

Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30887-1-AP

Print datasheet

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

验证数据展示

  • WB analysis using 30887-1-AP

    WB analysis using 30887-1-AP

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 30887-1-AP (Phospho-p130Cas / BCAR1 (Tyr410) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with p130Cas / BCAR1 antibody (16815-1-AP) subsequently.

经过测试的应用

Positive WB detected inpervanadate treated HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30887-1-AP targets Phospho-p130Cas / BCAR1 (Tyr410) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name breast cancer anti-estrogen resistance 1
Synonyms BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p130Cas, encoded by the breast cancer anti-estrogen resistance 1 (BCAR1) gene, is a scaffold protein that integrates large multi-protein complexes in response to stimuli such as hormones, growth factors, and integrin engagement. Upon environmental signals, Src family members and focal adhesion kinase (FAK) phosphorylate the p130Cas substrate domain (SD). This active p130Cas can recruit adaptor proteins such as Crk and Nck to its phosphorylated SD. (PMID: 34830244)

实验方案

Product Specific Protocols
WB protocol for Phospho-p130Cas / BCAR1 (Tyr410) antibody 30887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols