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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

    WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

    Non-treated NIH/3T3 cells, UV treated and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

  • WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

    WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

    Non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

Phospho-p38 MAPK (Thr180/Tyr182) Recombinant antibody, PBS Only

Phospho-p38 MAPK (Thr180/Tyr182) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81212-2-PBS

产品说明书

CloneNo. 242308D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

产品信息

81212-2-PBS targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 14
别名
计算分子量 360 aa, 41 kDa
观测分子量38-42 kDa
GenBank蛋白编号BC031574
基因名称 p38 MAPK
Gene ID (NCBI) 1432
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16539
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)