Phospho-p70(S6K) (Thr389) Polyclonal antibody

Phospho-p70(S6K) (Thr389) Polyclonal Antibody for WB, ELISA
Cat No. 28735-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, ELISA and More (1)

p p70(S6K) Thr389, p p70(S6K) (Thr389), p p70(S6K), p70 S6 Kinase (Thr389), p70(S6K) (Thr389)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inIGF-1 treated MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RPS6KB1 Ready-To-Use IHC Kit. RPS6KB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28735-1-AP targets Phospho-p70(S6K) (Thr389) in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 70kDa, polypeptide 1
别名 p p70(S6K) Thr389, p p70(S6K) (Thr389), p p70(S6K), p70 S6 Kinase (Thr389), p70(S6K) (Thr389)
观测分子量65-85 kDa
GenBank蛋白编号NM_003161
基因名称 p70 S6K
Gene ID (NCBI) 6198
RRIDAB_2918197
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23443
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70 S6K1 at T389 and p85 S6K1 at T412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)

实验方案

Product Specific Protocols
WB protocol for Phospho-p70(S6K) (Thr389) antibody 28735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Phytomedicine

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells

Authors - Maolei Xu
humanWB

Oxid Med Cell Longev

Alpha-Ketoglutarate Alleviates Neuronal Apoptosis Induced by Central Insulin Resistance through Inhibiting S6K1 Phosphorylation after Subarachnoid Hemorrhage

Authors - Peng-Fei Ding
humanWB

Mol Oncol

PMID: 29633504

Authors - Yi Xiao
humanWB

Aging (Albany NY)

UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.

Authors - Minfen Zhang
humanWB

Int J Mol Sci

Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

Authors - Juanli Ji
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