Phospho-p70(S6K) (Thr389) Polyclonal antibody

Phospho-p70(S6K) (Thr389) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28735-1-AP

Print datasheet

Synonyms

p70 S6 Kinase (Thr389), Phospho-p70, phospho-p70 S6 kinase, Phospho-p70 S6 Kinase (Thr389), Phospho-p70(S6K)



经过测试的应用

Positive WB detected inIGF-1 treated MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RPS6KB1 Ready-To-Use IHC Kit. RPS6KB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28735-1-AP targets Phospho-p70(S6K) (Thr389) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Synonyms p70 S6 Kinase (Thr389), Phospho-p70, phospho-p70 S6 kinase, Phospho-p70 S6 Kinase (Thr389), Phospho-p70(S6K)
Observed Molecular Weight65-85 kDa
GenBank Accession NumberNM_003161
Gene Symbol p70 S6K
Gene ID (NCBI) 6198
RRIDAB_2918197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70 S6K1 at T389 and p85 S6K1 at T412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)

实验方案

Product Specific Protocols
WB protocol for Phospho-p70(S6K) (Thr389) antibody 28735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Phytomedicine

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells

Authors - Maolei Xu
humanWB

Oxid Med Cell Longev

Alpha-Ketoglutarate Alleviates Neuronal Apoptosis Induced by Central Insulin Resistance through Inhibiting S6K1 Phosphorylation after Subarachnoid Hemorrhage

Authors - Peng-Fei Ding
humanWB

Mol Oncol

PMID: 29633504

Authors - Yi Xiao
humanWB

Aging (Albany NY)

UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.

Authors - Minfen Zhang
humanWB

Int J Mol Sci

Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

Authors - Juanli Ji