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验证数据展示

  • WB analysis using 29248-1-AP

    WB analysis using 29248-1-AP

    Non-treated MCF-7 cells, phosphatase inhibitor treated and λ phosphatase MCF-7 cells were subjected to SDS PAGE followed by western blot with 29248-1-AP (Phospho-p70(S6K) (Thr421/Ser424) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of HeLa using 29248-1-AP

    IF Staining of HeLa using 29248-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-p70(S6K) (Thr421/Ser424) antibody (29248-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Phospho-p70(S6K) (Thr421/Ser424) Polyclonal antibody

Phospho-p70(S6K) (Thr421/Ser424) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29248-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, P70S6K1, Phospho-p70(S6K) (Thr421/Ser424), PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated MCF-7 cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS6KB1 Ready-To-Use IHC Kit. RPS6KB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29248-1-AP targets Phospho-p70(S6K) (Thr421/Ser424) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Synonyms p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, P70S6K1, Phospho-p70(S6K) (Thr421/Ser424), PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A
Calculated Molecular Weight 59 kDa
Observed Molecular Weight65-85 kDa
GenBank Accession NumberBC053365
Gene Symbol p70 S6K
Gene ID (NCBI) 6198
RRIDAB_2935414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). In the conventional model, Thr421/Ser424 sites are phosphorylated before Thr389 phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) and subsequent Thr229 phosphorylation by PDK1. (PMID: 25100792, PMID: 24970012, PMID: 21602892)

实验方案

Product Specific Protocols
WB protocol for Phospho-p70(S6K) (Thr421/Ser424) antibody 29248-1-APDownload protocol
IF protocol for Phospho-p70(S6K) (Thr421/Ser424) antibody 29248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols