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验证数据展示

  • IHC staining of human kidney using 55463-1-AP

    IHC staining of human kidney using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human kidney using 55463-1-AP

    IHC staining of human kidney using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human lung cancer using 55463-1-AP

    IHC staining of human lung cancer using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human colon cancer using 55463-1-AP

    IHC staining of human colon cancer using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human colon using 55463-1-AP

    IHC staining of human colon using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included

Phospholipase C epsilon 1 Polyclonal antibody

Phospholipase C epsilon 1 Polyclonal Antibody for IHC, ELISA
Cat No. 55463-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue, human oesophagus cancer tissue, human stomach cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLCE1 Ready-To-Use IHC Kit. PLCE1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55463-1-AP targets Phospholipase C epsilon 1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, epsilon 1
Synonyms KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC
Calculated Molecular Weight 257 kDa
GenBank Accession NumberNM_016341
Gene Symbol PLCE1
Gene ID (NCBI) 51196
RRIDAB_3086452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P212
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase C epsilon 1 (PLCE1) is also named as KIAA1516, PLCE and PPLC. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. PLCE1, which mediates small GTPases of the Ras superfamily through the activity of its Ras guanine exchange factor, is a bifunctional enzyme (PMID: 31867717). As the effector of heterotrimer and small G protein, it is involved in regulating cell growth, cell survival, T-cell activation, and actin organization (PMID: 32238860). PLCE1 is identified as a member of phospholipase family, and PLCE1 was upregulated in LPS-induced pneumonia cells (PMID: 35527658). PLCE1 is involved in the regulation of Rho GTPases and MAPKs in podocytes, and PLCE1 interacts with NCK2 but not with NCK1 (PMID: 32238860). PLCE1 is a multifunctional enzyme exhibiting phospholipase as well as RasGEF activities (PMID: 11022047).

实验方案

Product Specific Protocols
IHC protocol for Phospholipase C epsilon 1 antibody 55463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols