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Phospholipase C epsilon 1 Polyclonal antibody

Phospholipase C epsilon 1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 55463-1-AP

Print datasheet

Synonyms

KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC

验证数据展示

  • IHC staining of human kidney using 55463-1-AP

    IHC staining of human kidney using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human kidney using 55463-1-AP

    IHC staining of human kidney using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human lung cancer using 55463-1-AP

    IHC staining of human lung cancer using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human colon cancer using 55463-1-AP

    IHC staining of human colon cancer using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included
  • IHC staining of human colon using 55463-1-AP

    IHC staining of human colon using 55463-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLCE1 antibody included

经过测试的应用

Positive IHC detected inhuman kidney tissue, human oesophagus cancer tissue, human stomach cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLCE1 Ready-To-Use IHC Kit. PLCE1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55463-1-AP targets Phospholipase C epsilon 1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, epsilon 1
Synonyms KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC
Calculated Molecular Weight 257 kDa
GenBank Accession NumberNM_016341
Gene Symbol PLCE1
Gene ID (NCBI) 51196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P212
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase C epsilon 1 (PLCE1) is also named as KIAA1516, PLCE and PPLC. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. PLCE1, which mediates small GTPases of the Ras superfamily through the activity of its Ras guanine exchange factor, is a bifunctional enzyme (PMID: 31867717). As the effector of heterotrimer and small G protein, it is involved in regulating cell growth, cell survival, T-cell activation, and actin organization (PMID: 32238860). PLCE1 is identified as a member of phospholipase family, and PLCE1 was upregulated in LPS-induced pneumonia cells (PMID: 35527658). PLCE1 is involved in the regulation of Rho GTPases and MAPKs in podocytes, and PLCE1 interacts with NCK2 but not with NCK1 (PMID: 32238860). PLCE1 is a multifunctional enzyme exhibiting phospholipase as well as RasGEF activities (PMID: 11022047).

实验方案

Product Specific Protocols
IHC protocol for Phospholipase C epsilon 1 antibody 55463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols