验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, Neuro-2a cells, SK-N-SH cells |
| Positive IHC detected in | human liver tissue, mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26205-1-AP targets FAM38B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | FAM38B fusion protein Ag24528 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | family with sequence similarity 38, member B |
| Synonyms | Piezo2, Transmembrane protein C18orf30, C18orf30 |
| Calculated Molecular Weight | 318 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | AB527139 |
| Gene Symbol | FAM38B |
| Gene ID (NCBI) | 63895 |
| RRID | AB_2880425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H5I5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.The 80 kDa band detected by SDS-PAGE can be caused by alternative splicing (PMID: 34335288, 37227654).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM38B antibody 26205-1-AP | Download protocol |
| IHC protocol for FAM38B antibody 26205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss | ||
Glia Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes | ||
Int J Biol Sci Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing. | ||
Adv Clin Exp Med PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway | ||
Cells Midpalatal Suture: Single-Cell RNA-Seq Reveals Intramembrane Ossification and Piezo2 Chondrogenic Mesenchymal Cell Involvement | ||
Front Mol Neurosci Activation of mechanoreceptor Piezo1 inhibits enteric neuronal growth and migration in vitro |
