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验证数据展示

  • WB analysis of human placenta using 66728-1-Ig

    WB analysis of human placenta using 66728-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66728-1-Ig (Placental lactogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 66728-1-Ig

    IHC staining of human placenta using 66728-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66728-1-Ig (Placental lactogen antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 66728-1-Ig

    IHC staining of human placenta using 66728-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66728-1-Ig (Placental lactogen antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Placental lactogen Monoclonal antibody

Placental lactogen Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66728-1-Ig

产品说明书

CloneNo. 1D11E4

宿主/亚型

Mouse / IgG3

种属反应性

human

应用

WB, IHC, ELISA

别名

CSH2, CSH1, CSB, CS 2, Choriomammotropin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66728-1-Ig targets Placental lactogen in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Placental lactogen fusion protein Ag9412 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 chorionic somatomammotropin hormone 2
别名 CSH2, CSH1, CSB, CS 2, Choriomammotropin
计算分子量 217 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC022044
基因名称 CSH2
Gene ID (NCBI) 1443
RRIDAB_2882078
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01243
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus. Both CSH1 and CSH2 can be detected by this antibody.

实验方案

Product Specific Protocols
WB protocol for Placental lactogen antibody 66728-1-IgDownload protocol
IHC protocol for Placental lactogen antibody 66728-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols