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Conjugate
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验证数据展示

  • WB analysis of mouse brain using 12555-1-AP (same clone as 12555-1-PBS)

    WB analysis of mouse brain using 12555-1-AP (same clone as 12555-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PrP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12555-1-AP (same clone as 12555-1-PBS)

    IP experiment of mouse brain using 12555-1-AP (same clone as 12555-1-PBS)

    IP result of anti-PrP (IP:12555-1-AP, 4ug; Detection:12555-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

    IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRNP antibody included
  • IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

    IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRNP antibody included
  • FC experiment of SH-SY5Y using 12555-1-AP (same clone as 12555-1-PBS)

    FC experiment of SH-SY5Y using 12555-1-AP (same clone as 12555-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PrP (12555-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

Prion protein PrP Polyclonal antibody, PBS Only

Prion protein PrP Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 12555-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

PRNP, PrP, Alternative prion protein, AltPrP, CD230

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRNP Ready-To-Use IHC Kit. PRNP primary antibody included.

产品信息

12555-1-PBS targets Prion protein PrP in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Prion protein PrP fusion protein Ag3257 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prion protein
别名 PRNP, PrP, Alternative prion protein, AltPrP, CD230
计算分子量 34 kDa
观测分子量 30 kDa
GenBank蛋白编号BC022532
基因名称 PrP
Gene ID (NCBI) 5621
RRIDAB_2237745
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDF7VJQ1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.